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Background:Multidrug resistance of tumor cells is one of the important factors that cause failure of chemotherapy in advanced gastric cancer. Tumor necrosis factor-related apoptosis-inducing ligand(TRAIL)may enhance the killing effect of chemotherapeutics on tumor cells,and reverse drug-resistant cell lines to sensitive cell lines,but its mechanism is not yet clear. Aims:To study the effect of TRAIL on expression of multidrug resistance gene glutathione S-transferase-π(GST-π)in drug-resistant human gastric cancer cell line SGC-7901 / VCR and the potential mechanism of TRAIL in reversing multidrug resistance of gastric cancer cells. Methods:SGC-7901 / VCR cells were treated with TRAIL in different doses (50,100,200 and 400 μg/ L)for 48 hours. After treatment,expression of GST-π mRNA in SGC-7901 / VCR cells and concentration of GST-π in culture supernatant were detected by RT-PCR and ELISA,respectively. Results:TRAIL could inhibit mRNA expression and protein secretion of GST-π in SGC-7901 / VCR cells in a dose-dependent manner within a certain range(≤200 μg/ L). The relative expression levels of GST-π mRNA in 50,100,200 and 400 μg/ L TRAIL groups were 0. 89 ± 0. 04,0. 77 ± 0. 08,0. 65 ± 0. 06 and 0. 61 ± 0. 03,respectively,and the concentrations of GST-π in culture supernatant in these groups were(57. 56 ± 1. 19)ng/ mL,(56. 30 ± 0. 80)ng/ mL,(31. 41 ± 1. 65)ng/ mL and (30. 80 ± 1. 34)ng/ mL,respectively,all were significantly lower than those in control group[1. 01 ± 0. 13 and(58. 62 ± 1. 38)ng/ mL,P all < 0. 05]. Conclusions:TRAIL may play a potential role in reversing multidrug resistance of gastric cancer cells through down-regulating GST-π expression.
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Background:Functional gastrointestinal disorders(FGIDs)is a common disease of digestive system which severely affects the quality of life. Recently,more and more attentions have been paid to the relationship between FGIDs and psychological factors. Aims:To investigate the relationship between FGIDs and psychological factors and related treatment. Methods:A total of 270 patients with FGIDs admitted from Sept. 2013 to Sept. 2014 at Anhui No. 2 Province People’s Hospital were enrolled. Hamilton Anxiety Scale(HAMA)and Hamilton Depression Scale(HAMD)were used to evaluate the mental state of patients,and symptom severity of digestive system was scored. Patients with psychological abnormalities received conventional therapy to relieve digestive symptoms,and those who failed from conventional therapy were assigned into refractory group and others were assigned into response group. Patients in refractory group were further divided into conventional therapy group and combined therapy group for receiving paroxetine in addition to conventional therapy. Results:The rates of anxiety and depression were 61. 5%(166 / 270)and 57. 0%(154 / 270),respectively. The scores of HAMA and HAMD were positively correlated with the score of digestive symptoms(r = 0. 585,P < 0. 01;r = 0. 584,P <0. 01)in patients with anxiety and depression in response group. The scores of HAMA,HAMD and digestive symptoms in response group after conventional therapy were significantly decreased(P < 0. 05),while the scores of HAMA and HAMD in refractory group after therapy were significantly higher than those in response group before therapy(P < 0. 05). The scores of HAMA,HAMD and digestive symptoms in combined therapy group were significantly lower than those in conventional therapy group(P < 0. 05). Conclusions:Relationship is found between FGIDs and psychological factors,and antidepressants might contribute to the amelioration of symptoms of FGIDs.
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Objective To analyze the clinical feature,the endoscopic treatment,therapy of complication of colorectal polyps.Methods According to polyps'size,location and shape in 157 patients with colorectal polyps,corresponding endoscopic treatment method was selected and observed the complication of the endoscopic treatment and therapeutic efficacy.Results 219 colorectal polyps were found in 157 patients.Colorectal polyps occurred frequently in rectum and sigmoid colon.Moreover,inflammatory polyps and hyperplastic polyp were the majority,and a small portion of polyps may turn cancerous.By using endoscopic therapy,hemorrhage was found in 12 samples,and the bleeding ratio was only 5.5％.Conclusion A diversity of methods used in endoscopic therapy of colorectal polyps is safe,efficient,resulting in less complications.
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<p><b>OBJECTIVE</b>To study chemical constituents of leaves from Discocleidion rufescens.</p><p><b>METHOD</b>Column chromatography and spectroscapic methods were used to isolate and identify the constituents.</p><p><b>RESULT</b>Fifteen compounds were isolated and identified as chrysophanol (1), physcione (2), taraxerol (3), beta-sitosterol (4), daucosterol (5), scopoletin (6), apigenin (7), acacetin-7-O-beta-D-glucoside (8), apigenin-7-O-beta-D-glucoside (9 ), luteolin (10), diosmetin-7-O-beta-D-glucoside (11), luteolin-7-O-beta-D-glucoside (12), gallic acid (13), amentoflavone (14) and myo-inositol (15).</p><p><b>CONCLUSION</b>Compounds 1, 2, 8, 11, 13 and 15 were isolated from the genus Discocleidion for the first time.</p>
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Euphorbiaceae , Chemistry , Flavones , Plant Extracts , SitosterolsABSTRACT
Lung resistance protein (LRP) is a gene which is found early and is closely related to multidrug resistance. LRP is expressed in most normal tissues with secretory function and is involved in secretion and cell detoxification function. LRP can also be seen expressed in malignant tumors including gastric cancer. There is a certain correlation between LRP expression and the prognosis of malignant tumor patients. LRP plays the role of multi-drug resistance mainly by preventing chemotherapy drugs into the nucleus and pumping chemother apy drugs out of the cells.
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Objective To investigate the effect of total flavonoid of Herba Pyrolae(TFHP) on the changes of ECG in rats with acute myocardial ischemia as well as the mechanism of the action.Methods Acute myocardial ischemic model was established by intravenous injection of pituitrin in rats,and ECG was recorded.Acute myocardial infarction model was established by the ligation of left descending coronary artery in rats,and serum level of nitric oxide(NO) and free fatty acid(FFA) was determined with spectrophotometrical method besides ECG.Results In comparison with the model controls,TFHP antagonized the decreased T wave by pituitrin and ligating coronary artery,and increased NO level and reduced FFA concentration in the serum of rats with acute myocardial ischemia.Conclusion TFHP protects the myocardium from ischemic injury possibly through increasing NO release and decreasing FFA production.
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Objective: To investigate the changes and significance of microvessel density(MVD) in bone marrow of patients with aplastic anemia. Methods: 30 aplastic anemia cases,20 hyperplastic anemia cases,and 20 normal individuals′ paraffin-embedded bone marrow biopsys were stained with HE and CD34 by immunohistochemistry method respectively;and the vol% of hematopoietic tissue area in bone marrows were detected accordingly. Results:The difference between the MVD of HE and CD34 was not significant in aplastic anemia cases ,and the difference between the MVD of HE and CD34 was not significant in normal individuals either. While the MVD of HE was significantly lower than that of CD34 in hyperplastic anemia cases. The difference of the MVD between light and heavy pathogenetic condition in aplastic anemia cases was significant.The MVD of CD34 were significantly different among the cases of aplastic anemia, hyperplastic anemia,and normal individuals.The MVD showed significant linear correlation with the vol% of hematopoietic tissue area (r=0.74,P